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We rst make note of the frontier orbitals Frontier Orbitals HOMO and LUMO The HOMO nO is a nonbonding MO strongly localized to oxygen It is predominantly a 2p orbital lying in the plane of the molecule Within the (local) C2v point group, it transforms as the b2 irreducible representation The LUMO pCO is a p antibonding orbital strongly polarized toward the carbon atom, and relatively low in energy, at least compared to the position of the pCC orbital of ethylene We know this because the C O 2p 2p p-type interaction matrix element hAB should be similar in magnitude to the interaction between 2p of carbon atoms, but the oxygen and carbon 2p orbital energies are quite di erent, leading to a smaller he U for pCO Within the (local) C2v point group, it transforms as the b1 irreducible representation Bonding.

6. Choose All Customization Files from the drop-down list. You now see AB2 listed when you expand the Partial CUI Files item. Double-click the AB2 item to expand it. 7. Right-click the AB2 item and choose New Menu. Enter AB2 to rename the menu. 8. To create a sub-menu, right-click AB2 and choose New Sub-menu. Enter &Special Edits to rename the sub-menu. (The ampersand allows you to open the menu by pressing Alt+S.) 9. To create the first custom menu item, click New in the Command List pane. In the Properties pane, enter the following:

Secondly, although large capacity HPLC columns are available, they are very expensive and small capacity analytical columns are much more common These latter columns place severe limitations on loadings achievable in HPLC (up to a maximum of approx 200 g, depending on the column) Pharmacia Biotech (currently GE Healthcare) pioneered Fast Protein Liquid Chromatography (FPLC) to address the particular needs of active biopolymer puri cation Although this chromatography format (Figure 223) was originally developed for protein puri cation, it has since been used for the puri cation of a wide variety of other biomolecules including DNA (plasmids, restriction fragments and oligonucleotides), nucleotide derivatives (eg NAD, ATP), carbohydrates as well as peptides and other low molecular weight biomolecules The system uses piston-driven, reciprocating pumps Each contains a pair of pistons operating in opposite directions (one lling as the other empties) which avoids pulse formation.

Of the four occupied MOs, two are bonding and two are nonbonding, resulting in a net bond order of 2, that is, a double bond Both the s and p bonds are polarized toward oxygen, the p more than the s because of the smaller intrinsic overlap (p-type overlap is smaller than s-type overlap) Dipole Moment A large bond dipole moment is expected, with the negative end at oxygen and the positive end at carbon Geometry The combination of s and p bonds forces coplanarity of the oxygen atom, the carbon atom, and the other two atoms attached to the carbon atom Ionization One cannot say anything about the magnitude of the ionization potential from just one interaction diagram.

Hydrogen bonding also underlies secondary structure in DNA and RNA and in DNA-RNA complexes. FTIR spectroscopy has found widespread use in the study of many biological processes involving nucleic acids. These studies range from in vitro experiments with synthetic oligonucleotides or their chemical analogues to investigations of tumour samples from cancer patients. The effect of nucleotide composition and sequence on base-pairing, the process of DNA-RNA hybridization and potential of DNA/RNA ratio in grading the severity of cancer in human patients have all recently been investigated.

However, we can say with con dence that the lowest energy (strictly speaking, the ground electronic state of the) molecular ion, M , would correspond to a radical cation localized to the oxygen atom With this foreknowledge, one would expect to nd mass spectral fragments which arise from the McLa erty rearrangement and from rupture of the bond to the carbonyl group:.